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目的了解广州管圆线虫Ⅰ期幼虫是否感染河蚬、河蚌,为研究广州管圆线虫病提供科学依据。方法从广州管圆线虫疫区龙海市海澄镇采集广州管圆线虫中间宿主褐云玛瑙螺,解剖分离Ⅲ期幼虫。大白鼠共12只,分3组,每组4只,经口感染大白鼠,每组分别感染100、50和25条广州管圆线虫Ⅲ期幼虫/只,40 d后采集鼠粪镜检,确认感染成功。将鼠笼置于实验感染池上方,以鼠粪便内广州管圆线虫Ⅰ期幼虫感染河蚬、河蚌。结果感染第14 d解剖河蚌和河蚬,均检出广州管圆线虫Ⅲ期幼虫,感染第28 d镜检,两者感染率均为100%。河蚌和河蚬感染度分别为162.3条/个、2.84条/g和6.9条/个、1.73条/g。三组感染大鼠于30 d、40 d、58 d的死亡率分别为100%、75%、25%。结论河蚬、河蚌可为广州管圆线虫适宜中间宿主。
Objective To understand whether stage Ⅰ larvae of Acanthomium canenella were infected with R. argenteus and R. musculus and provide a scientific basis for the study of C. elegans. Methods The A. noctua, an intermediate host of C. elegans, was collected from Haicheng Town, Longhai City, Guangzhou, and the stage Ⅲ larva was dissected. A total of 12 rats were divided into 3 groups, 4 rats in each group. Rats were infected by oral infection with 100, 50 and 25 Acremonium cercariae phase III larvae per mouse, respectively. Confirm the infection is successful. The squirrel cage was placed above the pool of experimental infection in order to infest the river larvae and mussels in the first stage larvae of A. cantonensis in the rat droppings. Results The third instar larvae of C. elegans were detected on the 14th day after infection, and the infection rate was 100% on the 28th day. The infection rates of mussels and tangs were 162.3 / a, 2.84 / g and 6.9 / a, 1.73 / g respectively. The mortality rates of the three groups of infected rats at 30 d, 40 d and 58 d were 100%, 75% and 25% respectively. Conclusions River clam and river clam can be suitable intermediate hosts for C. elegans.