论文部分内容阅读
目的:优化筛选牛蒡苷元的酶水解条件、提取工艺和大孔树脂型号,得到适合工业生产的工艺流程。方法:以时间、温度、溶媒量为考查因素,用单因素和正交试验设计法,对牛蒡苷元水解条件进行优化;以乙醇浓度、用量、提取次数、提取时间为因素,对提取方法优化;以吸附率和解吸附率对不同型号的大孔树脂优选。结果:牛蒡苷元最佳水解条件为42℃,12倍量水,水解12 h;最佳提取工艺为10倍量40%乙醇提取3次,每次0.5 h;AB-8大孔树脂纯化效果理想。结论:通过对牛蒡苷元的水解提取纯化工艺研究,确定了适合的工业生产的最佳工艺,该工艺方法简单方便,通过该工艺和进一步的硅胶柱层析分离,可得到纯度大于98%的牛蒡苷元。
Objective: To optimize the enzymatic hydrolysis conditions of arctigenin, the extraction process and the macroporous resin model to obtain the technological process suitable for industrial production. Methods: The time, temperature, and the amount of solvent were used as the investigation factors. The optimization of the arctigenin hydrolysis conditions was carried out by single factor and orthogonal experimental design. The optimum extraction conditions were ethanol concentration, amount, extraction times and extraction time ; Different types of macroporous resins are preferred for adsorption and desorption rates. Results: The optimal conditions of arctigenin hydrolysis were 42 ℃, 12 times of water and 12 hours of hydrolysis. The best extraction process was 10 times of 40% ethanol extraction for 3 times, each time being 0.5 h. The purification effect of AB-8 macroporous resin ideal. Conclusion: The optimum extraction process of arctigenin was determined by the method of hydrolytic extraction of arctigenin. The optimum conditions for the industrial production were determined. The method was simple and convenient. The purity of the arctigenin was more than 98% by this method and further silica gel column chromatography Arctigenin