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目的探讨反向寡核苷酸探针杂交技术(PCR-RDB)在人乳头状瘤病毒(HPV)分型检测中的临床应用。方法采用PCR-RDB对524例HPV感染的女性生殖道标本进行HPV分型检测,分析各型的感染率以及感染病毒与年龄段关系。结果 524例HPV感染者共检出5种低危型和18种高危型,其中单一型感染者304例,占58.02%,2种混合感染79例,占15.07%,3种混合感染51例,占9.73%,4种混合感染45例,占8.59%,5种混合感染35例,占6.68%,>5种混合感染10例,占1.91%,低危型感染以HPV-6、11为主,高危型感染以HPV-16、18、58、52、33型为主,不同年龄段感染各型也存在差异,15~25岁感染者以低危感染为主,多重感染主要发生在该年龄段;46~55岁年龄段感染高危型较多,随着宫颈糜烂程度的增加多重感染数量也增加。结论 PCR-RDB法可检测HPV多种亚型,对HPV感染早期诊断及预防宫颈病变和治疗具有较好的临床应用价值。
Objective To investigate the clinical application of Reverse Oligonucleotide Probe Hybridization (PCR-RDB) in the detection of human papillomavirus (HPV) typing. Methods PCR-RDB was used to detect HPV genotypes in 524 HPV-infected female genital tract specimens. The infection rate of each genotype and the relationship between the virus and age group were analyzed. Results A total of 524 cases of HPV infection were detected in 5 low-risk and 18 high-risk cases, of which 304 cases were single infection, accounting for 58.02%, 79 cases were mixed infection of 2 kinds, accounting for 15.07%, 51 cases were mixed infection of 3 kinds, Accounted for 9.73%, four mixed infections 45 cases, accounting for 8.59%, five mixed infections in 35 cases, accounting for 6.68%,> 5 mixed infections in 10 cases, accounting for 1.91%, low-risk infection with HPV-6,11 , High-risk type of infection is mainly HPV-16,18,58,52,33 type, there are differences in different types of infection of different ages, 15 to 25-year-old infected with low-risk infection, multiple infections occur mainly in this age Sections of 46-55 years of age infected with more high-risk type, with the increase of cervical erosion increased the number of multiple infections. Conclusion The PCR-RDB method can detect multiple subtypes of HPV, and has a good clinical value for early diagnosis of HPV infection and prevention of cervical lesions and treatment.