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以紫花苜蓿为试材,采用土培试验方法,研究了重金属Zn胁迫对紫花苜蓿体内丙二醛含量、脯氨酸含量、可溶性糖含量和可溶性蛋白质含量的影响及紫花苜蓿对Zn的积累能力。结果表明:胁迫23d时,随锌胁迫浓度的增加,紫花苜蓿不同器官中丙二醛含量、脯氨酸含量、可溶性糖含量和可溶性蛋白质含量增加,同时,紫花苜蓿叶片的锌积累量大于茎和根系的积累量。综合研究表明,在900μM Zn胁迫下,根据渗透调节物质的积累量,可初步判断紫花苜蓿有解毒和积累锌的能力,具有用于修复重金属Zn污染土壤的潜能。
Using alfalfa as test material, the effect of heavy metal Zn stress on malondialdehyde content, proline content, soluble sugar content and soluble protein content in alfalfa and the ability of alfalfa to accumulate Zn were studied by soil culture experiment. The results showed that the MDA content, soluble sugar content and soluble protein content increased in different organs of Medicago sativa at 23 d after stress, meanwhile, the accumulation of Zn in alfalfa leaves was greater than that of stem and Root accumulation. The comprehensive study showed that alfalfa could detoxify and accumulate zinc according to the accumulation of osmotic adjusting substances under the stress of 900μM Zn, which has the potential to repair Zn contaminated soil.