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为了解不同类型异质雄性不育小麦的易恢性差异和不同恢复系的恢复力差异,采用特异性分子标记及醇溶蛋白的A-PAGE检测方法对5种类型同核异质不育系(K706A、Va706A、Ju706A、C6706A、U706A,分别为K、Va、Ju、C6和U型)、保持系(706B)及恢复系(6521-2、LK783、1321、223原-9、9023晚、X197、宿7078、宿968、中国春、WM5-5)进行1B/1R类型鉴定,同时对不同杂交组合F1(不育系/恢复系)的自交结实率进行异地(杨凌和三原)分析。结果表明,Va706A、Ju706A、C6706A、U706A、706B为1BL/1RS易位纯合体,宿968、宿7078、LK783、1321、6521-2、WM5-5、X197、223原-9为非1BL/1RS易位材料,K706A、9023晚为1BL/1RS易位杂合体。5种细胞质雄性不育系中,K型的易恢性最好,Ju型和U型居中,C6型恢复能力最差。10个恢复系中,LK783和中国春在三原和杨凌恢复度均较高,宿968的恢复能力最弱,但LK783的变异幅度较大,中国春的变异幅度较小,说明中国春恢复能力好且稳定。此外,不育系与恢复系间存在明显的互作关系。
In order to understand the difference of recoverability and heterogeneity of restoring lines of heterogeneous genic male sterile wheat, A-PAGE method with specific molecular markers and gliadin (K706A, Va706A, Ju706A, C6706A, U706A, K, Va, Ju, C6 and Urespectively), maintainer line (706B) and restorer line (6521-2, LK783, X197, Sushu7078, Sinkou968, Chinese Spring, WM5-5) were identified by 1B / 1R. At the same time, inbred (Yangling and Mianyuan) analysis of selfed seed setting rate of F1 (CMS line / restorer line) was carried out in different hybrid combinations. The results showed that Va706A, Ju706A, C6706A, U706A and 706B were homozygous for 1BL / 1RS translocation, and 968, S-7078, LK783, 1321, 6521-2, WM5-5 and X197, 223- Translocation material, K706A, 9023 late 1BL / 1RS translocation hybrid. Of the five cytoplasmic male sterile lines, K type had the best recoverability, Ju type and U type were the center, and C6 type had the worst recovery ability. Among 10 restorer lines, LK783 and Chinese Spring had higher recovery degree in Sanyuan and Yangling, while Sink 968 had the weakest recovery ability, but LK783 had a larger variation range and smaller variation in spring in China, which indicated that spring resilience in China was good And stable. In addition, there is a clear interaction between male sterile line and restorer line.