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【目的】对3个枣疯病病原物泰安株系进行分子鉴定。【方法】采用植原体通用引物对R16F2n/R16R2,通过直接PCR技术,扩增枣疯病植原体16S rDNA基因,通过16S rDNA基因序列分析和在线模拟16S rDNA-RFLP分析,并将其16S rDNA基因序列提交到GenBank数据库。【结果】3个枣疯病病原物16S rDNA基因片段与16SrⅤ-B亚组中枣疯病植原体(AB052876和AF279272)、樱桃致死黄化植原体(AY197659)及杏卷叶植原体(FJ572660)的同源性高达99.5%99.7%,分别命名为枣疯病植原体泰安圆铃1号株系(Jujube witches’-broom phytoplasma strain Yuanling1,JWB-Yuanling1,TA)、枣疯病植原体泰安鲁北冬枣株系(Jujube witches’-broom phytoplasma strain Lubeidongzao,JWB-Lubeidongzao,TA)和枣疯病植原体泰安大白铃株系(Jujube witches’-broom phyto-plasma strain Dabailing,JWB-Dabailing,TA),基因登录号分别为:HM989946、HM989947和HM989948。【结论】3个枣疯病植原体泰安株系均归属于16SrⅤ-B亚组。
【Objective】 The molecular identification of three Tai’an lines with jujube bark disease was carried out. 【Method】 The 16S rDNA gene of 16 Phylogenetic tree was amplified by direct phytoplankton primer pair R16F2n / R16R2 and sequenced by 16S rDNA gene sequence and 16S rDNA-RFLP. The 16S rDNA gene The sequence was submitted to the GenBank database. 【Result】 The results showed that 16S rDNA gene fragments of three jujube broom pathogens were closely related to 16SrⅤ-B subgroups of jujube baker’s disease (AB052876 and AF279272), Echinochloa crus-galli (AY197659) and apophyllina (FJ572660) The homology was 99.5% and 99.7%, respectively, and named as Jujube witches’-broom phytoplasma strain Yuanling1 (TA), Zao bing phytoplasma strain Yuanling1 Jujube witches’-broom phytoplasma strain Lubeidongzao (JWB-Lubeidongzao, TA) and Jujube witches’-broom phyto-plasma strain Dabailing (TA) Accession numbers are: HM989946, HM989947 and HM989948. 【Conclusion】 The three Tai’an strains of jujube baker’s disease belong to 16SrⅤ-B subgroup.