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文章通过初步观察大肠癌患者癌组织与正常成人肠黏膜蛋白质的差异表达,为进一步探讨大肠的分子机制奠定基础。应用蛋白质组学的双向电泳技术,对大肠癌患者与正常成人肠黏膜蛋白质表达进行比较,观察其差异点。得到分辨率和重复性均好的大肠癌患者与正常成人直肠黏膜蛋白的双向凝胶电泳图谱,发现二者之间在表达上有明显差异,差异表达蛋白质点数共26个差异点,其中在大肠癌组织中表达上调点11个,下调的点15个。共分析了10个差异蛋白点,在10个蛋白质点中有5个蛋白质点得到鉴定。结论是大肠癌原发灶和正常肠黏膜蛋白质组表达有显著差异,HSP27蛋白、S100A9蛋白和GST-π蛋白表达上调,GRP75蛋白表达下调有可能与大肠癌发生相关。
By observing the differential expression of intestinal mucosal proteins in colorectal cancer patients and normal adults, the article lays the foundation for further exploration of the molecular mechanism of large intestine. The application of proteomics two-dimensional electrophoresis technology, colorectal cancer patients and normal intestinal mucosal protein expression were compared to observe the differences. The two-dimensional gel electrophoresis pattern of rectal mucosal protein in patients with colorectal cancer with good resolution and repeatability was found, and there was a significant difference between the two in rectal mucosal protein expression, with 26 differentially expressed protein spots differentially expressed in the large intestine Up-regulated in cancer tissues was 11 and 15 were down-regulated. Ten differential protein spots were analyzed, and five protein spots were identified in ten protein spots. The results showed that there was a significant difference in proteomic expression between primary colorectal cancer and normal intestinal mucosa. The expression of HSP27, S100A9 and GST-π were up-regulated. The down-regulation of GRP75 protein may be related to the occurrence of colorectal cancer.