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以三倍体枇杷茎尖为外植体,研究了取材时间及茎尖大小、植物生长调节剂对三倍体枇杷茎尖培养与植株再生过程中萌发、伸长、生根及移栽的影响。结果表明:春季取材且茎尖大小为0.7 cm时,茎尖成活率最高,为77.5%;初代培养适宜培养基为MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+IBA 1.5 mg/L,在此培养基上,三倍体枇杷茎尖萌发率高达91%;茎尖伸长适宜培养基为MS+6-BA 0.5 mg/L+NAA 0.3 mg/L+GA30.3 mg/L,平均芽苗高度可达4.9 cm;芽苗增殖适宜培养基为MS+6-BA 1.0 mg/L+NAA 0.3 mg/L+Tryptone 750 mg/L,增殖系数为7.7;生根适宜培养基为1/2MS+NAA 0.25 mg/L+IBA 2.0 mg/L,生根率为73.3%;三倍体枇杷组培苗移栽入腐熟有机肥∶园土∶锯末(1∶2∶1)的基质中成活率达到93.67%。
The triploid loquat shoot tips were used as explants to study the effects of time and shoot tip size, plant growth regulators on the culture of triploid loquat shoots, and the germination, elongation, rooting and transplanting of triploid loquat shoots. The results showed that the highest shoot apical survival rate of 77.5% was obtained in spring when the tip size was 0.7 cm and the best medium for primary culture was MS + 6-BA 1.0 mg / L + NAA 0.5 mg / L + IBA 1.5 mg / L, the germination rate of triploid loquat shoots was as high as 91% on this medium; the appropriate culture medium for stem elongation was MS + 6-BA 0.5 mg / L + NAA 0.3 mg / L + GA 30.3 mg / L , The average height of shoots was 4.9 cm. The optimum culture medium for shoots proliferation was MS + 6-BA 1.0 mg / L + NAA 0.3 mg / L + Tryptone 750 mg / L, and the multiplication coefficient was 7.7. / 2MS + NAA 0.25 mg / L + IBA 2.0 mg / L, and the rooting rate was 73.3%. The triploid loquat tissue culture seedlings were transplanted into the decomposed organic fertilizer: the soil of the garden: sawdust (1: 2: 1) Rate reached 93.67%.