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目的 探讨肝细胞癌 (HCC)组织中中期因子 (midkine ,MK)的表达及其与乙肝病毒(HBV)感染的关系。方法 采用原位杂交及免疫组织化学方法检测 6 2例HCC、癌旁肝组织及 10例正常肝组织中MKmRNA、MK蛋白表达及HBVDNA水平。结果 6 2例HCC组织中MKmRNA、MK蛋白表达阳性率分别为 74 2 %、75 8% ,明显高于无表达的癌旁肝组织及正常肝组织 (均P <0 0 1)。肝癌组织HBVDNA核型阳性率 (6 2 9% ,39/ 6 2 )明显高于癌旁肝组织 (6 5 % ,4 / 6 2 ) (P <0 0 1)。肝癌组织MKmRNA、MK蛋白表达与HBVDNA核型存在密切相关。结论 MKmRNA及其蛋白在HCC组织中呈过度表达 ,并可能与HBVDNA核型存在有密切联系
Objective To investigate the expression of midkine (MK) in hepatocellular carcinoma (HCC) and its relationship with hepatitis B virus (HBV) infection. Methods In situ hybridization and immunohistochemistry were used to detect the expressions of MKmRNA, MK protein and HBVDNA in 62 HCC tissues, 1 para-cancerous liver tissue and 10 normal liver tissues. Results The positive rates of MK mRNA and MK protein expression in 62 HCC tissues were 74 2% and 75 8%, respectively, which were significantly higher than those in non-expressed paracancer tissues and normal liver tissues (all P <0.01). The positive rate of HBVDNA karyotype (6 2 9%, 39/62) in hepatocellular carcinoma tissues was significantly higher than that in adjacent liver tissues (65%, 4/62) (P 0 01). The expression of MKmRNA and MK protein in HCC tissues is closely related to the existence of the karyotype of HBVDNA. Conclusion MKmRNA and its protein are overexpressed in HCC tissues and may be closely related to the existence of HBVDNA karyotype