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目的 探讨甲状腺转录因子 1(TTF 1)和表面活性蛋白A、B(SP A ,SP B)在肺硬化性血管瘤 (PSH)中表达的生物学意义。方法 用免疫组织化学的方法 ,检测 4 2例经外科手术切除、病理诊断的PSH标本中TTF 1、SP A、SP B和上皮膜抗原 (EMA)、波形蛋白 (vimentin)、广谱角蛋白(pancytokeratin)、角蛋白 7(CK7)、CK5 / 6、钙结合蛋白 (calretinin)、S 10 0蛋白、神经元特异性烯醇化酶(NSE)、突触素 (Syn)、嗜铬素A(CgA)、CD3 4 、Ⅷ因子相关抗原 (F Ⅷ )、平滑肌肌动蛋白 (SMA)的表达状况 ,其中 10例进行透射电镜观察。结果 病理组织学上肿瘤主要由多角形细胞和立方细胞组成 ;免疫组织化学显示 95 % (4 0 / 4 2 )患者立方细胞和多角形细胞共同表达TTF 1、88% (37/ 4 2 )表达EMA、5 7% (2 4 / 4 2 )表达vimentin ,93% (39/ 4 2 )患者立方细胞表达SP A、81% (34/ 4 2 )表达SP B、10 0 %(4 2 / 4 2 )表达 pancytokeratin ;2 4 % (10 / 4 2 )患者多角形细胞散在表达Syn、14 % (6 / 4 2 )表达NSE、12 %(5 / 4 2 )表达S 10 0和 12 % (5 / 4 2 )表达CgA ;TTF 1和EMA在这两种细胞之间的表达状况差异无显著性 (χ2 分别为 0 0 0 0和 3 4 0 3,P >0 0 5 ) ,vimentin在这两种细胞之间的表达差异有显著性 (χ2 =10 118,P <
Objective To investigate the biological significance of the expression of thyroid transcription factor 1 (TTF 1) and surfactant proteins A and B (SP A and SP B) in pulmonary sclerosing hemangioma (PSH). Methods Immunohistochemistry was used to detect the expression of TTF 1, SP A, SP B and epithelial membrane antigen (EMA), vimentin, broad-spectrum keratin (CK7), CK5 / 6, calretinin, S100 protein, neuron specific enolase (NSE), syn, synaptophysin (CgA ), CD3 4, factor Ⅷ related antigen (F Ⅷ) and smooth muscle actin (SMA) were detected in 10 cases. Transmission electron microscopy was performed in 10 cases. Results The histopathology showed that the tumors mainly consisted of polygonal cells and cuboidal cells. Immunohistochemistry showed that the expression of TTF in cuboidal and polygonal cells in 95% (40/42) patients was 1,88% (37/42) EMA showed vimentin in 57% (4/4 2), SP A in cubic cells (93% (39/42)), SP B in 81% (34/42) 2) expressed pancytokeratin. Synaptic cells were expressed diffusely in 24% (10/42) of patients, 14% (6/42) expressed NSE, 12% (5/4 2) expressed S 10% and 12% / 4 2) expression of CgA; TTF 1 and EMA expression in these two cells showed no significant difference (χ2 = 0 0 0 0 and 3 4 0 3, P> 0 0 5), vimentin in these two There was a significant difference in the expression of various kinds of cells (χ2 = 10 118, P <