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目的探讨N5,N10亚甲基四氢叶酸还原酶(MTHFR)基因多态性及血浆同型半胱氨酸(Hcy)与老年冠心病的相关性。方法应用高效液相色谱法和多聚酶链反应限制性内切酶片段长度多态性技术检测并比较了120例老年冠心病患者(CHD组)和58例健康老年人(对照组)的血浆Hcy浓度及MTHFR基因型。结果两组MTHFR 677位点基因型分布和各等位基因频率比较均有统计学差异(P均<0.05);CHD组T等位基因频率及血浆Hcy浓度高于对照组(P均<0.05)。结论 MTHFR基因突变可导致血浆Hcy浓度升高,高Hcy浓度及MTHFR基因T型均为老年CHD的高危因素。
Objective To investigate the association of N5, N10 methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and plasma homocysteine (Hcy) with coronary heart disease in the elderly. Methods High-performance liquid chromatography (HPLC) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were used to detect and compare plasma concentrations of Hcy in 120 elderly patients with coronary heart disease (CHD) and 58 healthy controls And MTHFR genotypes. Results There were significant differences in genotype distribution and allele frequencies between MTHFR 677 locus and control group (P <0.05) . Conclusion Mutation of MTHFR gene leads to the increase of plasma Hcy concentration. High Hcy concentration and MTHFR gene T-type are risk factors of CHD in the elderly.