论文部分内容阅读
目的了解广西空肠弯曲菌和结肠弯曲菌引起腹泻的流行病学特点以及在健康人群和动物中携带状态。方法在广西崇左市大新县和南宁市对腹泻病例、5岁以下健康人群、养猪场和屠宰厂职工以及养殖场和农贸市场的猪、鸡等进行流行病学调查并采集粪便标本,进行实验室空弯菌和结弯菌的分离培养、PCR鉴定以及脉冲场凝胶电泳分型(PFGE)分析。结果弯曲菌检出率为2.13%(21/985),空肠弯曲菌和结肠弯曲菌检出率分别为1.12%(11/985)和1.02%(10/985)。腹泻病例检出率为0.89%(6/677),以5岁以下年龄组和20～29岁年龄组检出阳性率为高,除冬季外其余季节均检出阳性;人群弯曲菌检出阳性率为8.26%(10/121),2岁以下空肠弯曲菌检出率较高,结肠弯曲菌则在每个年龄段均有检出;鸡、猪检出弯曲菌阳性率为3.23%(5/155),空肠弯曲菌和结肠弯曲菌的检出率分别为1.29%(2/155)和1.94%(3/155),两种动物的阳性率差异无统计学意义。所有生化结果阳性的菌株其PCR复核鉴定结果均为阳性。来自南宁市和大新县的两株空弯菌PFGE型别一致,提示二者可能存在相同的感染源。结论广西地区人群及部分动物中空肠弯曲菌、结肠弯曲菌的流行状况呈现多样化和复杂化态势。
Objective To understand the epidemiological characteristics of Campylobacter jejuni and Campylobacter coli in Guangxi and its status in healthy population and animals. Methods Daxin County and Nanning City, Guangxi Zhuang Autonomous Region epidemiological investigation of diarrhea cases, healthy population under 5 years of age, pig farm and slaughter plant workers and farms and farmers markets pigs and chickens were collected and stool specimens were collected, Isolation, culture and PCR identification of Meloidogyne spp. And Meloidogyne spp. In laboratory were analyzed by PFGE. Results The positive rate of Campylobacter was 2.13% (21/985). The positive rates of Campylobacter jejuni and Campylobacter coli were 1.12% (11/985) and 1.02% (10/985), respectively. The detection rate of diarrhea cases was 0.89% (6/677). The detection rate was high in the age group of 5 years old and in the age group of 20 ~ 29 years old, and was positive in the rest of the seasons except in winter. The prevalence of Campylobacter was positive The prevalence of Campylobacter jejuni was lower than 2 years old and Campylobacter coli was detected in all age groups. The positive rate of Campylobacter spp. Detected in chickens and pigs was 3.23% (5) / 155). The positive rates of Campylobacter jejuni and Campylobacter coli were 1.29% (2/155) and 1.94% (3/155), respectively. There was no significant difference in the positive rates between the two animals. All biochemical positive strains confirmed by PCR test results were positive. Two strains of Campylobacter PFGE from Nanning City and Daxin County were consistent, suggesting that they may share the same source of infection. Conclusions The prevalence of Campylobacter jejuni and Campylobacter coli in Guangxi population and some animals shows diversification and complication.