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复膜氧电极用来检测酶活性和植物光合作用中氧变化已有报道。由于氧电极检测的原理是根据氧分压的变化,而与被测溶液的物理状况,如澄清度、粘度、颜色等无关。因此,本文报道的实验方法适用范围广,有一定的实用价值。本文以过氧化氢酶(Catalase 简称CTS)为例,讨论用复膜氧电极测定释氧酶反应速率的实验条件。用本法测定产氧速率有方法简便、结果直观、可自动记录等优点,是当今国内外研究和测定释氧酶活性的新方法。材料和方法1、复膜氧电极仪复膜氧电极仪由以下几部分组成:复膜氧电极及溶氧控制仪(由中国科学院上海植物生理研究所制造),自动记录仪(由上海大华仪表厂生产);酶反应室(由中国科学院上海生物化学研究所提供),见图1。
Fumo oxygen electrode used to detect enzyme activity and oxygen changes in plant photosynthesis have been reported. As the oxygen electrode detection principle is based on changes in oxygen partial pressure, and the physical state of the solution under test, such as clarity, viscosity, color and so on. Therefore, the experimental methods reported in this paper have a wide range of applications and have certain practical value. In this paper, catalase (Catalase referred to as CTS), for example, to discuss with the membrane oxygen electrode assay of the oxygenase reaction rate experimental conditions. The determination of oxygen production rate with this method is simple, intuitive and can automatically record the advantages of research and determination of the current domestic and foreign activities of a new method of oxygenase activity. Materials and methods 1. Fumo oxygen electrode instrument Fumo oxygen electrode instrument consists of the following parts: Fumo oxygen electrode and dissolved oxygen controller (by the Chinese Academy of Sciences, Shanghai Institute of Plant Physiology), automatic recorder (from Shanghai Dahua Instrument Factory production); enzyme reaction chamber (provided by the Shanghai Institute of Biochemistry, Chinese Academy of Sciences), see Figure 1.