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目的:建立祛风解毒颗粒中芍药苷的HPLC含量测定方法。方法:采用色谱柱:Agilent C18(250 mm×4.6 mm,5μm);流动相:乙腈-水(16∶84);流速:1.0 mL.min-1;检测波长:230 nm;柱温:30℃。结果:HPLC法测芍药苷在0.025 78~0.515 5mg.mL-1范围内,线性关系良好,样品平均回收率为100.2%,RSD为1.25%。结论:HPLC法测定祛风解毒颗粒中芍药苷的含量方法可行,重复性良好,测定结果可靠,可用于祛风解毒颗粒的质量控制。
Objective: To establish a HPLC method for the determination of paeoniflorin in Qufeng Jiedu Granules. Methods: The column was Agilent C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-water (16:84), the flow rate was 1.0 mL.min-1. The detection wavelength was 230 nm. . Results: The linearity of paeoniflorin in the range of 0.025 78-0.515 5 mg.mL-1 was good with the average recovery of 100.2% and the RSD of 1.25%. Conclusion: HPLC method for determination of Paeoniflorin in Qufeng Jiedu granules method is feasible and reproducible, the determination of the results is reliable and can be used for the quality control Qufeng Jiedu particles.