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用抗白粉病的普通小麦一簇毛麦6VS/6AL易位系与普通小麦品种扬麦5号、普通小麦一簇毛麦6V代换系和中国春6A双端二体以及6V代换系和6A双端二体配制了4个测交组合,分析了这4个杂交组合F1PMC’sMIC-分带的减数分裂构型。在(6VS/6AL易位系×扬麦5号)和(6VS/6AL易位系×6V代换系)的F1PMC’sMI,分别观察到由易位染色体与6A染色体和6V染色体配对形成的具有特定C-分带带型的棒状二价体,杂种中的棒状二价体数目高于各自亲本中的棒状二价体数。在(易位系×C.S.d.d.t6A)F1中,在87.9%的PMC中观察到由易位染色体长臂与6AL端体配对形成的异形二价体(tl”)。而在(6V代换系×C.S.d.d.t6A)F1中,96.68%的PMC具有两个单价端体(t’,t’)。该结果进一步证实易位涉及簇毛麦染色体6VS和小麦染色体6AL,易位断点靠近着丝粒。在减数分裂中,易位染色体的正常配对和分离,保证了6VS上白粉病抗性基因Pm21的正常传递,为这一新抗源在小麦育种中的应用奠定了细胞学基础。
The results showed that 6VS / 6AL translocation line of common wheat cultivars resistant to powdery mildew and common wheat cultivars Yangmai 5, 6V substitution lines of common wheat cultivars and 6D substitution lines of Chinese Spring 6A and substitution lines of 6V 6A double-ended body was prepared four cross combinations, analysis of the four hybrid combinations F1PMC’sMIC-banding meiosis configuration. In the F1PMC’sMI of (6VS / 6AL translocation line × Yangmai 5) and (6VS / 6AL translocation line × 6V substitution line), the transformants formed by the pairing of the translocation chromosome with the 6A chromosome and the 6V chromosome were observed The number of rod-shaped divalent bodies in a particular C-banding type rod-like bivalents is higher than the number of rod-like divalent bodies in their respective parents. In the (translocation line × C.S.d.d.t6A) F1, a heterogeneous divalent body (tl ") formed by pairing of the translocation chromosome long arm with the 6AL terminal body was observed in 87.9% of PMC , While 96.68% of PMCs had two monovalent terminals (t ’, t’) in (6V substitution line × C.S.d.d.t6A) F1. This result further confirmed that the translocation involved clusters 6VS of maize chromosome and 6AL of wheat chromosome, the breakpoint of the translocation is close to the centromere. In meiosis, the normal mating and segregation of the translocated chromosomes ensure the normal transmission of powdery mildew resistance gene Pm21 on 6VS The application of new anti-source in wheat breeding laid the foundation for cytology.