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我们曾用脂肪酸自旋标记化合物I(10,3)研究过抗癌药物硫杂脯氨酸(THIPRO),阿糖胞苷(CYTO)、放线菌素D(ACTI)和5-氟-2′-脱氧尿嘧啶(5-F-U)对中国地鼠肺正常细胞V_(79)和癌变细胞V_(79)-B_1膜脂流动性的影响,发现这几种药物都使细胞膜脂有序度变大,流动性变小,其中以硫杂脯氨酸对细胞膜表层作用最明显。为了弄清这几种抗癌药物对膜的作用机理,我们采用一种卵磷脂人工膜体系,用抗坏血酸(V_c)还原自旋标记动力学探讨了这几种抗癌药物对膜的通透性的影响,并且观察了加入白蛋白对上述影响的作用。
We have studied the anticancer drugs THIPRO, CYTO, ACTI, and 5-Fluoro-2 using the fatty acid spin-labeled compound I (10,3) ’- deoxyuracil (5-FU) on Chinese hamster lung normal cells V_ (79) and cancerous cells V_ (79) -B_1 membrane fluidity and found that these drugs have the degree of cellular membrane lipid change Large, less mobility, of which thioproline on the cell membrane surface most obvious role. In order to clarify the mechanism of action of these anticancer drugs on the membrane, we used a lecithin artificial membrane system to investigate the permeability of these anticancer drugs to the membrane with ascorbic acid (V_c) reduction spin labeling kinetics The effects of addition of albumin on these effects were also observed.