论文部分内容阅读
应用Simonsen的脾指数测定方法以及体内生成脾结节和体外琼脂培养方法,分别研究了小鼠脾脏和外周血中造血干细胞和免疫活性细胞在自然沉降条件下的沉降特性。在小鼠脾脏细胞的沉降试验中,CFU-S的沉降速度为4.50毫米/小时,CFU-C的沉降速度为6.69毫米/小时,脾指数阳性细胞的沉降速度为3.57毫米/小时。在小鼠外周血白细胞的沉降试验中,CFU-S的沉降速度为4.96毫米/小时,脾指数阳性细胞的沉降速度为5.12毫米/小时。上述沉降试验表明,造血干细胞与免疫活性细胞有不同的沉降速度和分布特性,因此,有可能通过自然沉降,在一定程度上分开这两类细胞,其中,粒系定向造血干细胞(CFU-C)的沉降速度要高于多向性造血干细胞(CFU-S),因而,在沉降分离中CFU-C与免疫活性细胞可以得到较高程度的分离。从造血细胞中分离免疫活性细胞是当前造血干细胞移植中的重要研究课题。本文对于应用速度沉降装置分离免疫活性细胞、减轻造血细胞移植中的移植物抗宿主反应(GVHR)的前景作了讨论。
The application of Simonsen spleen index method and in vivo spleen nodules and in vitro agar culture methods were studied in mice spleen and peripheral blood hematopoietic stem cells and immune cells in the sedimentation conditions of natural sedimentation characteristics. In the sedimentation assay of mouse spleen cells, the sedimentation rate of CFU-S was 4.50 mm / hr, that of CFU-C was 6.69 mm / hr, and that of spleen index positive cells was 3.57 mm / hr. In the mouse peripheral blood leukocyte sedimentation test, the sedimentation rate of CFU-S was 4.96 mm / hr, and the spleen index-positive cells settled at 5.12 mm / hr. The above sedimentation experiments show that hematopoietic stem cells have different sedimentation rate and distribution characteristics with immunocompetent cells. Therefore, it is possible to separate these two types of cells by natural sedimentation to a certain extent. Among them, granulocyte-directed hematopoietic stem cells (CFU-C) Sedimentation rate is higher than that of multidrug-derived hematopoietic stem cells (CFU-S), so CFU-C and immunocompetent cells can be separated to a higher degree in sedimentation separation. Isolation of immunocompetent cells from hematopoietic cells is an important research topic in current hematopoietic stem cell transplantation. This article discusses the potential of using velocity sedimentation to isolate immunocompetent cells and to reduce graft-versus-host reaction (GVHR) in hematopoietic cell transplantation.