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目的:建立同时测定气滞胃痛颗粒中白芍药苷、芍药苷、柚皮苷和甘草酸含量的高效液相色谱法。方法:采用AgilentZorbax SB-C18柱(250mm×4.6mm,5μm),柱温30℃;流动相为水(0.2%醋酸)-乙腈(0.2%醋酸),线性梯度洗脱,流速为1.0mL·min-1;检测波长为230nm(0~15min),284nm(15~20min),254nm(20~35min)。结果:白芍药苷、芍药苷、柚皮苷、甘草酸浓度分别在15.1~226μg.mL-1(r=0.9998),25.6~383μg.mL-1(r=0.9998),27.7~277μg.mL-1(r=0.9998),9.43~47.2μg.mL-1(r=0.9998)范围内线性关系良好。低、中、高3个浓度的平均加样回收率分别为97.4%~101%(RSD为0.8%~1.7%),96.8%~99.7%(RSD为1.4%~2.6%),97.3%~100%(RSD为1.9%~2.4%)。结论:本方法简便、准确,重复性好,可以为气滞胃痛颗粒的质量控制提供依据。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of the contents of Bletilla striol, paeoniflorin, naringin and glycyrrhizic acid in Qizhiweitong granules. METHODS: Agilent Zorbax SB-C18 column (250mm×4.6mm, 5μm) was used. The column temperature was 30°C. The mobile phase consisted of water (0.2% acetic acid)-acetonitrile (0.2% acetic acid). The linear gradient eluted at a flow rate of 1.0 mL·min. -1; Detection wavelength was 230 nm (0 to 15 min), 284 nm (15 to 20 min), and 254 nm (20 to 35 min). Results: The concentrations of paeoniflorin, paeoniflorin, naringin and glycyrrhizin were 15.1 to 226 μg.mL-1 (r=0.9998), 25.6 to 383 μg·mL-1 (r=0.9998), and 27.7 to 277 μg.mL-, respectively. The linearity was good in the range of 1 (r=0.9998) and 9.43~47.2μg.mL-1 (r=0.9998). The average recovery rates for low, medium, and high concentrations were 97.4% to 101% (RSD 0.8% to 1.7%), 96.8% to 99.7% (RSD 1.4% to 2.6%), and 97.3% to 100%. % (RSD is 1.9%~2.4%). Conclusion: The method is simple, accurate and reproducible, and can provide a basis for the quality control of Qizhiweitong granules.