论文部分内容阅读
目的研究饲料钙不足对大鼠血糖血脂的影响并对其可能的机制进行探讨。方法 SD雄性大鼠20只(7周龄),适应性喂养1周后按体重随机分为2组,即对照组(n=10,饲料含钙量0.5%)、钙不足组(n=10,饲料含钙量0.15%)。每日记录摄食量,每周称量体重,第16周末处死大鼠,采集血清测定血糖(glucose,GLU)、总胆固醇(total cholestrol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)和高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C);采集骨骼肌检测PGC-1αmRNA和蛋白的表达水平并分析其与血糖血脂的相关性。结果与对照组相比,钙不足组大鼠摄食量及体重均无差别(均有P>0.05),但血清GLU、TC、LDL-C水平升高,HDL-C/LDL-C比值降低(均有P<0.05),TG和HDL-C虽没有统计学差异但是有升高趋势;钙不足组大鼠骨骼肌内调控糖脂代谢及线粒体功能的关键因子过氧化物酶体增殖物激活受体γ辅激活因子-1α(proliferator-activated receptorγcoactivator-1α,PGC-1α)mRNA和蛋白的表达水平均降低(均有P<0.05)。大鼠骨骼肌PGC-1α表达水平与TC、LDL-C等水平呈负相关(均有P<0.05)。结论饲料钙不足虽未影响大鼠体重,但可通过抑制PGC-1α的表达从而引起大鼠血糖血脂紊乱。
Objective To study the effect of dietary calcium insufficiency on blood glucose and blood lipid in rats and to explore its possible mechanism. Methods Twenty male Sprague-Dawley rats (7 weeks old) were randomly divided into two groups according to body weight after one week of adaptive feeding: control group (n = 10, dietary calcium 0.5%), calcium deficiency group , Feed calcium content 0.15%). The rats were sacrificed at the end of the 16th week. Serum glucose, total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL-C) and high-density lipoprotein cholesterol (HDL-C). The skeletal muscle was collected to detect the expression of PGC-1αmRNA and protein and its correlation with blood glucose and blood lipid . Results Compared with the control group, there was no significant difference in food intake and body weight between the calcium deficient group and the control group (all P> 0.05), but the level of serum GLU, TC and LDL-C increased and the ratio of HDL-C / LDL-C decreased (P <0.05). TG and HDL-C showed no statistical difference, but increased with the increase of TG and HDL-C. Calcium-deficient rats regulate the regulation of glucose and lipid metabolism and mitochondrial function in skeletal muscle. Peroxisome proliferator-activated receptor The expression of PGC-1α mRNA and protein were decreased (both P <0.05). The expression of PGC-1α in skeletal muscle of rats was negatively correlated with the levels of TC and LDL-C (all P <0.05). Conclusion Although dietary calcium deficiency does not affect the body weight of rats, it can cause the disorder of blood glucose and blood lipid in rats by inhibiting the expression of PGC-1α.