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以苹果品种嘎拉试管苗顶部前３片展开叶为试材，将叶片由叶尖至叶基分为上、中、下３个区，每区又由叶脉分隔为左、右两个亚区，共６个亚区。每一叶片在１个亚区中心部位用解剖针刺伤１点后接种于ＭＳ＋ＢＡ１ｍｇ／Ｌ＋ＮＡＡ４ｍｇ／Ｌ＋２，４Ｄ０５ｍｇ／Ｌ＋蔗糖２０ｇ／Ｌ的培养基上，暗培养７ｄ后，转接到ＭＳ＋ＢＡ１ｍｇ／Ｌ＋蔗糖２０ｇ／Ｌ的培养基上继续暗培养，４０ｄ后，８５％的叶片在刺伤口周围发生直接类型体细胞胚胎。用４％海藻酸钠和２％氯化钙对体细胞胚胎进行人工种皮包裹后制成人工种子，在无菌条件下，人工种子转变为绿苗。
Taking the top 3 leaves of the top of apple plantlet Gala as the test material, the leaves were divided into upper, middle and lower leaves from leaf tip to leaf base. Each leaf was divided into left and right subregions , A total of six sub-regions. Each leaf was dissected on the medium of MS + BA1mg / L + NAA4mg / L + 2,4D05mg / L + sucrose 20g / L after being stabbed at the center of 1 sub-region by dissection needle for 1 hour. After dark culture for 7 days, MS + BA1mg / L + sucrose 20g / L on the medium to continue dark culture, 40d, 85% of the leaves in the stab wound around the direct type of somatic embryogenesis. Artificial seeds were made from artificial seed coat wrapped with 4% sodium alginate and 2% calcium chloride. Under sterile conditions, artificial seeds were transformed into green shoots.