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1989年建立以丙肝病毒(HCV)非结构区重组肽C_(100)为抗原的第一代抗-HCV检测方法,因其敏感性不够可能导致对HCV感染率估计过低。后来加入另一个非结构区重组抗原C_33和HCV核壳区抗原建立了第二代抗-HCV检测方法,提高试验敏感性。该文作者以四种免疫学方法(第一代EIA和中和试验、第二代EIA和免疫印迹试验)检测人类免疫缺陷病毒(HIV)感染病人中抗-HCV阳性率,探讨HIV对HCV感染的影响及HCV的传播。研究对象为法国多中心前瞻性研究中的272例HIV血清学阳性至少一年以上的患者,男174例、女98例,平均年龄30.9岁(18～78岁)。因静脉毒瘾而感染者97例(35.7％)、同性恋男子68例(25.0％)、
In 1989, a first-generation anti-HCV assay using HCV nonstructural peptide C_ (100) as an antigen was established. Insufficient sensitivity may lead to underestimation of HCV infection. Later joined another non-structural domain recombinant antigen C_33 and HCV core-shell antigen to establish a second-generation anti-HCV detection method to improve the test sensitivity. The authors tested the positive rate of anti-HCV in patients with human immunodeficiency virus (HIV) infection by four immunological methods (first-generation EIA and neutralization test, second-generation EIA and Western blot test) to investigate the effect of HIV on HCV infection The impact and spread of HCV. A total of 272 HIV-seropositive HIV-positive patients in the French multicentre prospective study were 174 males and 98 females with a mean age of 30.9 years (18-78 years). 97 (35.7%) were infected with venous addiction, 68 (25.0%) with homosexual men,