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目的探讨轮状病毒对乳鼠肠道外损害及Cp G ODN的保护作用。方法 24只肠道外轮状病毒感染乳鼠随机平均分为3组:对照组(生理盐水处理)、感染组(腹腔注射轮状病毒)、Cp G ODN组(连续腹腔注射50μg Cp G ODN后腹腔注射轮状病毒悬液);处理前后检查各组心、肝、肺组织改变,HRV VP7 mRNA水平以及心肝功能。结果感染组乳鼠心、肝、肺组织出现严重病理学改变,且心、肝组织HRV VP7 mRNA呈阳性,肺组织呈阴性。Cp G ODN组乳鼠心、肝、肺组织病理学改变较轻,心、肝组织HRV VP7 mRNA为弱阳性,肺组织为阴性。Cp G ODN组乳鼠FS、EF显著高于感染组但显著低于对照组,ALT、AST和LVESD显著低于感染组但显著高于对照组差异均有统计学意义(P<0.05)。结论腹腔注射轮状病毒损伤了乳鼠心、肝、肺组织及心肝功能;Cp G ODN预处理减轻了轮状病毒的损伤,对乳鼠具有保护作用。
Objective To investigate the protective effect of rotavirus on parenteral damage and CpG ODN in neonatal rats. Methods Twenty-four neonates with extravasculature rotavirus infection were randomly divided into three groups: control group (normal saline group), infection group (intraperitoneal injection of rotavirus) and CpG ODN group (continuous intraperitoneal injection of 50 μg CpG ODN) Injection of rotavirus suspension). Changes of heart, liver and lung tissue, HRV VP7 mRNA level, and heart and liver function were examined before and after treatment. Results Serum, liver and lung tissues of infected rats developed severe pathological changes, and heart and liver tissue HRV VP7 mRNA was positive and lung tissue was negative. The histopathological changes of heart, liver and lung of CpG ODN group rats were mild, HRV VP7 mRNA was weakly positive in heart and liver tissue, and lung tissue was negative. The FS and EF of the CP G ODN group were significantly higher than those of the infected group but significantly lower than those of the control group. The levels of ALT, AST and LVESD in the CP G ODN group were significantly lower than those in the infected group but significantly higher than those in the control group (P <0.05). Conclusion Intraperitoneal injection of rotavirus injures the heart, liver and lung tissues and heart and liver function of neonatal rats. Pretreatment with CpG ODN can reduce the damage of rotavirus and protect the neonatal rat.