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用Southern blot技术对乙肝病毒感染肝脏和外周血白细胞的情况进行了对比研究,发现白细胞的阳性率为44％,肝组织的阳性率为46％,两者无统计学差异。根据琼脂糖凝胶电泳速度可将病毒DNA分子分为游离型、整合型和混合型,配对检查两者亦无明确相关性,说明外周血白细胞是乙肝病毒攻击的靶细胞,亦是病毒复制的场所。白细胞中病毒DNA游离型与血清学指标HBeAg(+),HBV DNA斑点杂交(+)关系密切,而整合型除与上述指标有关外,还和抗-HBe(+)有关,表明病毒在乙肝发病过程中复制和部分静止的差异。
Southern blot analysis of hepatitis B virus infection in the liver and peripheral white blood cells were compared and found that the positive rate of white blood cells was 44%, the positive rate of liver tissue was 46%, no significant difference between the two. According to the speed of agarose gel electrophoresis virus DNA molecules can be divided into free-type, integrated and mixed type, there is no clear correlation between the paired test, indicating that peripheral blood leukocytes is the target of hepatitis B virus attack, is also a viral replication place. The leucocyte free DNA and serological markers HBeAg (+), HBV DNA dot blot (+) are closely related to, and in addition to the integration of the above indicators, but also anti-HBe (+), indicating that the virus in the pathogenesis of hepatitis B Differences in the process of replication and partial static.