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Ginsenoside compound K(GCK), the main metabolite of protopanaxadiol constituents of Panax ginseng, easily produces alkali metal adduct ions during mass spectrometry particularly with lithium. Accordingly, we have developed a rapid and sensitive liquid chromatography–tandem mass spectrometric method for analysis of GCK in human plasma based on formation of a lithium adduct. The analyte and paclitaxel(internal standard) were extracted from 50 m L human plasma using methyl tertbutyl ether. Chromatographic separation was performed on a Phenomenex Gemini C18 column(50 mm 2.0 mm; 5 μm) using stepwise gradient elution with acetonitrile–water and 0.2 mmol/L lithium carbonate at a flow rate of 0.5 m L/min. Detection was performed in the positive ion mode using multiple reaction monitoring of the transitions at m/z 629-449 for the GCK-lithium adduct and m/z 860-292 for the adduct of paclitaxel. The assay was linear in the concentration range 1.00–1000 ng/m L(r~2>40.9988)with intra- and inter-day precision of ±8.4% and accuracy in the range of 4.8% to 6.5%. Recovery,stability and matrix effects were all satisfactory. The method was successfully applied to a pharmacokinetic study involving administration of a single GCK 50 mg tablet to healthy Chinese volunteers.
Ginsenoside compound K (GCK), the main metabolite of protopanaxadiol constituents of Panax ginseng, easily produce alkali metal adducts ions during mass spectrometry particularly with lithium. in human plasma based on formation of a lithium adduct. The analyte and paclitaxel (internal standard) were extracted from 50 mL Human plasma using methyl tertbutyl ether. Chromatographic separation was performed on a Phenomenex Gemini C18 column (50 mm 2.0 mm; 5 μm ) using stepwise gradient elution with acetonitrile-water and 0.2 mmol / L lithium carbonate at a flow rate of 0.5 mL / min. Detection was performed in the positive ion mode using multiple reaction monitoring of the transitions at m / z 629-449 for the GCK-lithium adduct and m / z 860-292 for the adduct of paclitaxel. The assay was linear in the concentration range 1.00-1000 ng / m L (r ~ 2> 40.9988) with intra- and in ter-day precision of ± 8.4% and accuracy in the range of 4.8% to 6.5%. Recovery, stability and matrix effects were all satisfactory. The method was successfully applied to a pharmacokinetic study involving administration of a single GCK 50 mg tablet to healthy Chinese volunteers.