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目的:分析在荧光原位杂交技术慢性淋巴细胞白血病遗传学异常检测中的应用,并分析相关指标在评价患者预后中的应用。方法:对我院收治的45例初诊CLL患者采用荧光原位杂交技术进行特异性探针D13S25(13q14.3)、RB1(13q14)、p53(17p13)、ATM(11q22.3)、以及CSP12(12号染色体3体)染色体标本检测,分析CLL患者遗传学异常的发生率。采用实时定量PCR检测miR-15a和miR-16-1与CLL患者遗传学异常的相关性。结果:45例CLL初诊患者中,荧光原位检测发现CLL遗传学异常37例,CLL遗传学异常率82.22%。其中d(13q14.3)遗传异常13例,d(13q14)遗传异常7例,d(11q22-23)遗传异常6例,d(17p13)遗传异常5例,12号染色体三体异常6例,遗传学异常多呈异质性。实时定量PCR检测发现miR-15a和miR-16-1与d(13q14)遗传异常显著相关。结论:荧光原位杂交技术是一种检测CLL遗传学异常的快速、灵敏方法,可以提高CLL遗传异常检出率。miR-15a和miR-16-1可以预测d(13q14)遗传异常CLL患者预后。
Objective: To analyze the application of fluorescence in situ hybridization in the detection of genetic abnormality of chronic lymphocytic leukemia, and to analyze the application of relevant indexes in evaluating the prognosis of patients. Methods: Forty-five newly diagnosed CLL patients admitted to our hospital were detected by fluorescence in situ hybridization. The specific probes D13S25 (13q14.3), RB1 (13q14), p53 (17p13), ATM (11q22.3), and CSP12 Chromosome 3 on Chromosome 12), and analyzed the incidence of genetic abnormalities in CLL patients. The correlation between miR-15a, miR-16-1 and genetic abnormalities in CLL patients was examined by real-time PCR. Results: Of the 45 newly diagnosed CLL patients, 37 cases of CLL genetic abnormalities and 82.22% of CLL genetic abnormalities were detected by fluorescence in situ test. Among them, there were 13 cases of genetic abnormality of d (13q14.3), 7 cases of genetic abnormality of d (13q14), 6 cases of genetic abnormality of d (11q22-23), 5 cases of genetic abnormality of d (17p13), 6 cases of abnormal chromosome 3 trisomy 12, Generic abnormalities were heterogeneous. Real-time quantitative PCR detection found that miR-15a and miR-16-1 and d (13q14) genetic abnormalities were significantly correlated. Conclusion: Fluorescent in situ hybridization (FISH) is a rapid and sensitive method to detect genetic abnormalities in CLL, which can improve the detection rate of CLL genetic abnormalities. miR-15a and miR-16-1 Predict Prognosis in Patients with Genetic Abnormal d (13q14) CLL.