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目的:检测男性乳腺癌患者血清中VEGF及外周血CK19的表达水平,探讨二者的相互关系及临床意义。方法:应用ELISA法分别检测18例男性乳腺癌患者及8例健康男性志愿者血清中VEGF的水平,同时抽取相应的外周血标本,应用实时定量逆转录-聚合酶链反应(qRT-PCR)方法检测标本中CK19的表达水平,并进行相关性分析。结果:VEGF在男性乳腺癌患者血清中的表达水平明显高于正常健康男性,二者差异显著(P=0.015);亚组分析表明,在腋淋巴结阳性的患者中,血清VEGF水平明显高于淋巴结阴性患者(P=0.026)。CK19在健康男性外周血标本中未见表达,在男性乳腺癌患者外周血中,阳性表达5例(27.8%),而外周血中CK19阳性的男性乳腺癌患者血清VEGF水平显著高于CK19阴性的患者(130.34±77.18pg/ml vs58.01±52.43pg/ml,P=0.038)。结论:男性乳腺癌患者血清VEGF水平与外周血微转移密切相关,提示VEGF可能参与了肿瘤细胞的侵袭和播散,可作为男性乳腺癌早期血行播散的血清学指标。
Objective: To detect the serum levels of VEGF and CK19 in male patients with breast cancer, and to explore their correlation and clinical significance. Methods: The serum levels of VEGF in 18 cases of male breast cancer patients and 8 healthy male volunteers were detected by ELISA. At the same time, corresponding peripheral blood samples were collected and analyzed by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) The expression of CK19 in the specimens was detected and the correlation analysis was performed. Results: The expression of VEGF in serum of male patients with breast cancer was significantly higher than that of healthy men (P = 0.015). Subgroup analysis showed that serum VEGF levels were significantly higher in patients with axillary lymph nodes than those in lymph nodes Negative patients (P = 0.026). CK19 expression was not found in healthy male peripheral blood samples, 5 cases (27.8%) were positive in the peripheral blood of male patients with breast cancer, while the serum levels of VEGF in CK19-positive male breast cancer patients were significantly higher than those in CK19-negative Patient (130.34 ± 77.18 pg / ml vs 58.01 ± 52.43 pg / ml, P = 0.038). Conclusion: The serum level of VEGF in male patients with breast cancer is closely related to micrometastasis of peripheral blood, suggesting that VEGF may be involved in the invasion and dissemination of tumor cells, which may serve as a serological marker for hematogenous dissemination in early stage of male breast cancer.