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目的:研究喉鳞状细胞癌与喉良性病变组织中碳酸酐酶9(CAⅨ)和P糖蛋白(P-gp)表达的差异,分析2种蛋白在喉鳞状细胞癌组织中表达的相关性,及两者与喉鳞状细胞癌临床病理特征的关系。方法:采用免疫组织化学EnVision二步法分别检测47例喉鳞状细胞癌组织、20例声带息肉组织中CAⅨ和P-gp的表达情况。结果:CAⅨ和P-gp在喉鳞状细胞癌组织中的阳性表达率和它们在声带息肉中的表达均差异有统计学意义(P<0.05),且两者在喉鳞状细胞癌组织中的表达呈正相关(r=0.324,P<0.05)。CAⅨ蛋白表达与喉鳞状细胞癌的临床分期、淋巴结转移有关(P<0.05);与喉鳞状细胞癌组织学分级、原发部位无关(P>0.05)。P-gp表达与喉鳞状细胞癌的临床分期、组织学分级有关(P<0.05);与喉鳞状细胞癌的原发部位、淋巴结转移无关(P>0.05)。结论:CAⅨ与P-gp的高表达可能在喉鳞状细胞癌的病情进展中起一定作用。
OBJECTIVE: To investigate the differences of the expression of carbonic anhydrase 9 (CAIX) and P-glycoprotein (P-gp) between laryngeal squamous cell carcinoma and benign laryngeal lesions, and to analyze the correlation between the expression of two proteins in laryngeal squamous cell carcinoma , And their relationship with clinicopathological features of laryngeal squamous cell carcinoma. Methods: The expressions of CAⅨ and P-gp in 47 cases of laryngeal squamous cell carcinoma and 20 cases of vocal cord polyps were detected by immunohistochemistry EnVision two-step method. Results: The positive rates of CAⅨ and P-gp in laryngeal squamous cell carcinoma and their expression in vocal cord polyp were significantly different (P <0.05), and both of them in laryngeal squamous cell carcinoma (R = 0.324, P <0.05). The expression of CAⅨ protein was correlated with the clinical stage and lymph node metastasis of laryngeal squamous cell carcinoma (P <0.05), but not with the histological grade and primary site of laryngeal squamous cell carcinoma (P> 0.05). The expression of P-gp was correlated with the clinical stages and histological grade of laryngeal squamous cell carcinoma (P <0.05). The expression of P-gp was not associated with the primary site of laryngeal squamous cell carcinoma and lymph node metastasis (P> 0.05). Conclusion: The high expression of CAⅨ and P-gp may play a role in the progression of laryngeal squamous cell carcinoma.