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为探讨广州地区丙型肝炎病毒（ＨＣＶ）感染的基因型及其与疾病程度、感染途径和干扰素疗效的关系，作者应用分型ＰＣＲ技术对１５６例抗－ＨＣＶ阳性患者血清ＨＣＶＲＮＡ进行分型检测，并分析不同程度肝病及不同感染途径ＨＣＶ基因型分布，且对５１例经干扰素治疗的慢性丙肝作分型评价。结果１２４例ＨＣＶＲＮＡ阳性中，Ⅱ型占９０．３％（１１２例），Ⅲ型占８．１％（１０例），Ⅱ／Ⅲ型混合感染占１．６％（２例），未检出Ⅰ、Ⅳ型，说明广州地区ＨＣＶ感染以Ⅱ型为主；不同程度肝病及不同感染途径之间ＨＣＶ基因型构成比无明显差异，表明基因型与疾病严重程度及感染途径关系不大；干扰素治疗病例，ＨＣＶ－Ⅱ、Ⅲ型感染的总应答数、完全应答数及部分应答数分别为１８／４１，１１／４１，７／４１和８／１０，６／１０，２／１０，提示Ⅲ型感染疗效较好，ＨＣＶ基因型似可作为选择干扰素治疗病例及判断疗效的参考指标，但是由于观察的Ⅲ型病例数较少，需积累更多的资料才能作出更客观的结论。
To investigate the genotypes of hepatitis C virus (HCV) infection in Guangzhou and its relationship with disease severity, route of infection and efficacy of interferon, genotyping PCR was used to detect the serum HCVRNA in 156 anti-HCV positive patients , And analyzed the distribution of HCV genotypes in different degrees of liver disease and different infection routes, and evaluated 51 cases of chronic hepatitis C treated with interferon. Results Among the 124 HCV RNA positive cases, type Ⅱ accounted for 90.3% (112 cases), type Ⅲ accounted for 8.1% (10 cases), type Ⅱ / Ⅲ mixed infections accounted for 1.6% (2 cases), not detected Type I and Type IV, indicating that HCV infection in Guangzhou is mainly Type II; There is no significant difference in HCV genotype composition between different degrees of liver disease and different infection routes, indicating that the genotype is not related to the severity of the disease and the route of infection. Interferon The total response, complete response and partial response were 18/41, 11/41, 7/41 and 8/10, 6/10, 2/10 respectively for HCV-Ⅱ and type Ⅲ infections in treatment cases, suggesting that Ⅲ Type of infection is better, HCV genotypes may be used as a reference index for the selection of interferon treatment and efficacy, but due to the small number of type Ⅲ observed, more data accumulation in order to make more objective conclusions.