背景 HDN及输血反应均涉及Rb血型系统。一个逆转录病毒系统曾被用于表达携带RHCE基因编码的RH血型抗原肽链。本研究致力于C抗原的结构。材料与方法 将编码Ce抗原的全长度cDNA转入K562细胞,用单抗在流式细胞仪上检测C、e、E抗原表达。C抗原在K562细胞中表达前,发生了Cys16→Trp点突变的编码Ce和CE的cDNA检测C抗原上重要的Cys16。结果 转染的K562细胞产生了C、e抗原,与抗e与抗C Background HDN and transfusion reactions involve the Rb blood group system. A retroviral system was used to express the RH blood group antigen peptide chains that carry the RHCE gene. This study is committed to the structure of C antigen. MATERIALS AND METHODS Whole-length cDNA encoding Ce antigen was transferred into K562 cells and the expression of C, e and E antigens was detected by flow cytometry with monoclonal antibody. Prior to the expression of C antigen in K562 cells, cDNAs encoding Ce and CE that had a Cys16-> Trp point mutation occurred detected Cys16, which is important on the C antigen. Results The transfected K562 cells produced C, e antigens, with anti-e and anti-C