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从我国自行建株的人食道癌细胞系CaEs－17野生型中分离出两株放射抗拒的亚系克隆7和克隆10，其SF2值分别为0．41、0．58和0．61。存活曲线的分析表明，与野生型比较，克隆7及克隆10的Do值差异不大，但DQ与N值有较大的差别。野生型细胞系DQ为1．07Gy，而克隆7的DQ为1．85Gy，克隆10的DQ值为2．04Gy；野生型细胞的N值为1．96，克隆7亚系的N值为3．07，克隆10亚系的N值为3．63。用SouthernBlot杂交检测了H－ras基因的扩增，结果发现克隆10亚系在照射2Gy后H－ras显著扩增，可能克隆10的放射抗拒与H－ras的扩增有一定关系，但未发现两者之间有恒定的关系。
Two strains of radiation-resistant subclone clone 7 and clone 10 were isolated from the human esophageal cancer cell line CaEs-17 wild-type strain with self-built strains in China, and the SF2 values were 0.41, 0.58, and 0.61, respectively. The survival curve analysis showed that compared with the wild type, the Do values of clone 7 and clone 10 were not significantly different, but the DQ and N values were significantly different. The DQ of the wild-type cell line was 1.07 Gy, while the DQ of clone 7 was 1.85 Gy, the DQ value of clone 10 was 2.04 Gy; the N value of wild-type cells was 1.96, and the N value of clone 7 sub-line was 3 In .07, the N value of the clone 10 subline was 3.63. The amplification of H-ras gene was detected by SouthernBlot hybridization. It was found that H-ras was significantly amplified after irradiation of 2Gy in clone 10 sub-genome. It was possible that the radioresistance of clone 10 was related to the amplification of H-ras, but it was not found. There is a constant relationship between the two.