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目的:通过观察加味百合地黄汤对肝癌H22小鼠增殖细胞核抗原(PCNA)蛋白表达的影响。探讨其干预肿瘤生长的作用机理,为治疗肿瘤提供理论依据和客观指标。方法:ICR小鼠随机分为6组:正常组,模型组,化疗组,中药低、中、高剂量组,并对后5组进行肝癌H22肿瘤造模。用药前后取血,取脾、胸腺、瘤块等组织称重。15天后处死小鼠,免疫组化法检测瘤组织中PCNA蛋白表达情况。结果:中药高剂量组和化疗组PCNA蛋自表达呈弱阳性(±),中药高剂量组,化疗组与模型组比较,有显著性差异(P<0.01):中药高剂最组与化疗组比较,无显著性差异(P>0.05)。结论:加味百合地黄汤能降低PCNA蛋白的表达,可抑制肿瘤细胞增殖,对防治肿瘤生长有积极意义。
Objective: To observe the effects of flavored Lily Dihuang decoction on the expression of proliferating cell nuclear antigen (PCNA) protein in H22 mice with hepatocellular carcinoma. Explore the mechanism of its intervention in tumor growth mechanism for the treatment of tumors provide a theoretical basis and objective indicators. Methods: ICR mice were randomly divided into 6 groups: normal group, model group, chemotherapy group, low, medium and high dose Chinese medicine group, and the latter 5 groups were made HCC tumor model. Blood before and after treatment, take the spleen, thymus, tumor mass and other organizations weighing. The mice were sacrificed after 15 days and the expression of PCNA protein in the tumor tissue was detected by immunohistochemistry. Results: The expression of PCNA protein in high dose group and chemotherapy group was weakly positive (±). There was significant difference between high dose group and chemotherapy group (P <0.01) No significant difference (P> 0.05). Conclusion: Modified Lily Dihuang Decoction can reduce the expression of PCNA protein, inhibit the proliferation of tumor cells and have a positive effect on the prevention and treatment of tumor growth.