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目的探讨荧光原位杂交(FISH)检测在尿脱落细胞染色体畸变中的意义及与膀胱肿瘤病理分期的关系。方法用2组DNA探针CSP3/CSP7分别杂交于3号和7号染色体的着丝粒区域,GLPp16/CSP17分别杂交于9号染色体长臂(9q21)和17号染色体的着丝粒区域,检测36例疑似膀胱肿瘤患者血性尿液中的脱落细胞,与常规尿脱落细胞学检查比较,分析与肿瘤分期的关系。结果 FISH检测总敏感度为73.33%,总特异度为88.46%,总敏感度高于尿细胞学检测(26.70%);肌层浸润敏感度(88.24%)高于非肌层浸润敏感度(53.85%),二者分别高于尿细胞学检测(35.29%,15.38%),差别显著(P<0.05);3、7、9和17号染色体的畸变率分别为88.34%、64.71%、70.59%和88.24%,3号和17号染色体的畸变程度与肿瘤浸润深度呈正相关性(P<0.05)。结论 FISH检测尿脱落细胞诊断膀胱肿瘤优于常规尿脱落细胞学检查,对于有肌层浸润的膀胱肿瘤意义更明显,3号和17号染色体的畸变与肿瘤浸润深度有关。
Objective To investigate the significance of fluorescence in situ hybridization (FISH) detection in chromosomal aberration of exfoliated urothelial cells and its relationship with pathological stage of bladder cancer. Methods Two pairs of DNA probes, CSP3 / CSP7, were hybridized to the centromeric regions of chromosomes 3 and 7, respectively, and the GLPp16 / CSP17 was hybridized to the centromeric region of chromosome 9 long chromosome 9q21 and chromosome 17, respectively 36 cases of suspected bladder cancer patients with bloody urine exfoliated cells compared with conventional urine cytology, analysis and tumor staging relationship. Results The total sensitivity of FISH was 73.33%, the total specificity was 88.46%, the total sensitivity was higher than that of urinary cytology (26.70%). The sensitivity of myometrial invasion (88.24%) was higher than that of non-myometrial invasion (53.85% %), Which were higher than that of urine cytology (35.29%, 15.38%, respectively) (P <0.05). The aberration rates of chromosomes 3, 7, 9 and 17 were 88.34%, 64.71% and 70.59% And 88.24%. The degree of chromosome aberration on chromosomes 3 and 17 was positively correlated with the depth of tumor invasion (P <0.05). Conclusion The detection of exfoliated urothelial cells by FISH is superior to routine urine exfoliative cytology in diagnosis of bladder cancer, which is more significant for bladder tumor with myometrial infiltration. The aberrations of chromosomes 3 and 17 are related to the depth of tumor invasion.