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目的:证实抗表皮生长因子受体单克隆抗体耦联中空金纳米球(anti-EGFR/HGNs)增加宫颈癌细胞对HGNs的选择性摄取,并增强宫颈癌细胞的放射敏感性。方法:电化学置换法制备HGNs,透射电子显微镜(TEM)观察HGNs的尺寸和形态,电感耦合等离子体质谱(ICP-MS)检测细胞内HGNs摄取量,CCK-8法进行细胞毒性检测,流式细胞术(FCM)检测细胞周期,Western blot法检测Bcl-2、Bax、Bad、active caspase-3等凋亡相关蛋白的表达。结果:HGNs平均直径(54.6±7.11)nm,壁厚(5.01±2.23)nm。Anti-EGFR/HGNs与细胞共同孵育可致细胞内HGNs摄取量显著增加,anti-EGFR/HGNs联合照射亦表现出显著细胞毒性。Anti-EGFR/HGNs使处于G2/M期的宫颈癌细胞比率增加,并通过下调Bcl-2表达及上调Bax、Bad、caspase-3表达促进细胞凋亡。结论:anti-EGFR/HGNs能增加宫颈癌细胞对HGNs的摄取,并增强兆伏高能照射对细胞的毒性,提高细胞的放射敏感性。
OBJECTIVE: To demonstrate that anti-epidermal growth factor receptor monoclonal antibody conjugated hollow-gold nanoparticles (anti-EGFR / HGNs) increase the selective uptake of HGNs by cervical cancer cells and enhance the radiosensitivity of cervical cancer cells. Methods: HGNs were prepared by electrochemical method. The size and morphology of HGNs were observed by transmission electron microscopy (TEM). The uptake of HGNs by ICP-MS was measured. Cytotoxicity was detected by CCK-8. The cell cycle was detected by flow cytometry (FCM). The expression of Bcl-2, Bax, Bad and active caspase-3 were detected by Western blot. Results: The mean diameter of HGNs was (54.6 ± 7.11) nm and the wall thickness was 5.01 ± 2.23 nm. Anti-EGFR / HGNs co-incubated with cells caused a significant increase in intracellular HGNs uptake, and combined radiation with anti-EGFR / HGNs also showed significant cytotoxicity. Anti-EGFR / HGNs increased the proportion of cervical cancer cells in G2 / M phase and promoted apoptosis by down-regulating Bcl-2 expression and up-regulating Bax, Bad and caspase-3 expression. CONCLUSION: Anti-EGFR / HGNs can increase the uptake of HGNs by cervical cancer cells and enhance the cytotoxicity of mega-dose high-energy radiation and enhance the radiosensitivity of cells.