采用脂质体(liposomes)作为基因载体,具有毒性低、无免疫原性、易于制备及稳定性好的优点。已有将之应用于临床基因治疗试验的成功报道。存在的问题是转移基因在靶细胞核中表达效率不高。为此,作者设计了一种可以提高表达效率的胞浆表达系统。 由脂质体运载的转移基因表达效率不高的原因可能系靶细胞浆内溶酶体对脂质体-DNA复合物的 The use of liposomes as gene carriers has the advantages of low toxicity, no immunogenicity, easy preparation and good stability. It has been successfully applied in clinical gene therapy trials. The problem is that the expression of the transgene in the target cell nucleus is inefficient. To this end, the authors devised a cytoplasmic expression system that increases the efficiency of expression. The reason for the low efficiency of expression of the transgene carried by the liposomes may be due to the intracellular lysosomal lysosomal targeting of liposome-DNA complexes