论文部分内容阅读
目的观察缺血预适应对膨胀离体大鼠心室所致心律失常的影响。方法采用Langendorff方法灌流离体大鼠心脏。将可充灌液体的乳胶球囊通过左心房及二尖瓣置于左心室,通过向乳胶球囊注射液体对左室进行膨胀,记录膨胀前和膨胀过程中左室心电图和左室压力、冠脉流量和心率;并计算对照组和各实验组由膨胀诱发心律失常的发生率和持续时间。结果通过乳胶球囊膨胀左室,在左室舒张末压增加相同的情况下,缺血预适应组较对照组室性早搏和室性心动过速发生率均降低,持续时间显著缩短;加入维拉帕米、白屈菜赤碱或格列本脲后,室性早搏和室性心动过速发生率均增高,持续时间延长;单纯维拉帕米、白屈菜赤碱或格列本脲组心律失常发生情况和对照组相似。结论对大鼠心室进行膨胀可诱发心律失常;缺血预适应对心律失常的发生具有抑制作用;细胞内钙离子浓度的短暂升高可能是缺血预适应发挥抑制作用的驱动因子;ATP敏感性钾通道及蛋白激酶C在缺血预适应过程中可能发挥了重要作用。
Objective To observe the effect of ischemic preconditioning on ventricular arrhythmias induced by swell in isolated rats. Methods Langendorff method was used to perfuse isolated rat heart. The fluid-inflatable latex balloon was placed in the left ventricle through the left atrium and mitral valve, the left ventricle was inflated by injecting liquid into the latex balloon, the left ventricular pressure and left ventricular pressure were recorded before and during expansion, Pulse rate and heart rate. The incidence and duration of inflation-induced arrhythmia in control group and experimental group were calculated. Results Inflation of left ventricle through latex balloon left ventricular end-diastolic pressure increase in the same circumstances, ischemic preconditioning group compared with the control group, premature ventricular tachycardia and ventricular tachycardia incidence were reduced, the duration was significantly reduced; add Vera Pamivacaine, chelerythrine, or glyburide, the incidence of ventricular premature beats and ventricular tachycardia increased, the duration of prolongation; simple verapamil, chelerythrine or glibenclamide group heart rhythm The incidence of abnormalities and control group similar. CONCLUSION: Ventricular arrhythmia can be induced by swelling the ventricles of rats. Preconditioning with ischemic preconditioning can inhibit arrhythmia. A transient increase of intracellular calcium concentration may be the driving factor of ischemic preconditioning. ATP sensitivity Potassium channels and protein kinase C may play an important role in ischemic preconditioning.