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目的:探讨陕西省宝鸡地区妇女人群人乳头瘤病毒(HPV)感染亚型分布特征。方法:采用聚合酶链反应(PCR)反向点杂交技术对HPV进行分型检测。结果:宝鸡地区妇女人群HPV总感染率为35.92%,高危、低危、高低危感染率分别为24.27%、9.21%和2.44%。单一、高危单一和高危多重感染率分别为28.54%20.51%和3.76%。在20岁以下和60岁以上年龄段均有较高感染率。高危型感染率在大于60岁年龄段最高。单一感染常见亚型为16、6、58、11、53、52型;多重感染常见亚型为16、11、6、58、51、53型;总体感染常见亚型为16、6、58、11、53、52型;分别占本组感染71.58%、60.47%和66.86%。不同年龄段妇女人群常见亚型,≤20岁为58、6、39、11、16、56型,21~30岁为6、16、11、52、58、39型,31~40岁为16、6、58、11、52、53型,41~50岁为16、6、58、11、52、51型,51~60岁为16、6、53、11、58、42型,>60岁为16、6、58、11、53、56型;分别占本组感染76.86%、74.12%、71.33%、65.65%、60.92%和73.57%。在多重感染中以二重感染最为多见(5.67%),占多重感染的76.89%。单一、多重和总体HPV16亚型分别占24.34%、13.06%和20.04%。结论:陕西省宝鸡地区妇女人群HPV感染率较高,其常见高危亚型为16、58、53、52、39、56型,低危型为6和11型。不同年龄段亚型分布各有不同。总体、高危、低危和高低危感染率在不同年龄段均有显著差异。
Objective: To investigate the distribution of human papillomavirus (HPV) subtypes in women in Baoji region of Shaanxi province. Methods: Polymerase chain reaction (PCR) dot blot hybridization was used to detect HPV. Results: The total HPV infection rate of women in Baoji area was 35.92%. The infection rates of high-risk, low-risk and high-low risk were 24.27%, 9.21% and 2.44% respectively. Single, high-risk single and high-risk multiple infection rates were 28.54% 20.51% and 3.76%. In 20 years of age and above the age of 60 have a higher infection rate. High-risk infection rate in the more than 60 years of age the highest. The common subtypes of single infection were 16,6,58,11,53,52, the common subtypes of multiple infections were 16,11,6,58,51,53, the common subtype of total infection was 16,6,58, 11,53,52 type respectively accounted for 71.58%, 60.47% and 66.86% in this group. Common subtypes of women of different age groups, ≤ 20 years old 58,6,39,11,16,56 type, 21 to 30 years old 6,16,11,52,58,39 type, 31 to 40 years old as 16 , 6,58,11,52,53 type, 41 to 50 years old 16,6,58,11,52,51 type, 51-60 years old 16,6,53,11,58,42 type,> 60 Aged 16,6,58,11,53,56 type; accounted for this group were infected 76.86%, 74.12%, 71.33%, 65.65%, 60.92% and 73.57%. The most common infection with multiple infections (5.67%), accounting for 76.89% of multiple infections. Single, multiple and overall HPV16 subtypes accounted for 24.34%, 13.06% and 20.04%, respectively. Conclusion: The prevalence of HPV infection in Baoji district of Shaanxi province is high. The common high-risk subtypes are 16,58,53,52,39,56, and the low-risk types are 6 and 11. Different age distribution of subtypes are different. Overall, high-risk, low-risk and high-risk infection rate in different age groups were significantly different.