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目的：建立一种检测胎盘功能的新手段。方法：从人胎盘匀浆中分离、纯化出人胎盘泌乳素（ Ｈ Ｐ Ｌ）纯品，制备 Ｈ Ｐ Ｌ 抗体。用改良的过碘酸钠法制备 Ｈ Ｐ Ｌ抗体与辣根过氧化物酶结合物，建立了双抗体夹心的酶联免疫方法，检测了正常妊娠和高危妊娠孕妇血中 Ｈ Ｐ Ｌ水平。结果：高危妊娠和血中 Ｎ Ｐ Ｌ 值低，且 Ｈ Ｐ Ｌ 低值者的胎儿宫内窘迫、低 Ａｐｇａｒ 评分的发生率均高于 Ｈ Ｐ Ｌ 值正常组，两组相比差异均有显著性。结论：该法具有特异、敏感、快速、准确等优点，可作为检测胎盘功能的一项可靠指标。
Objective: To establish a new measure of placental function. Methods: Human placental lactogen (H P L) purified product was isolated and purified from human placenta homogenate to prepare H P L antibody. Hp L antibody and horseradish peroxidase conjugate were prepared by modified sodium periodate method. ELISA method of double antibody sandwich was established to detect the level of H P L in normal and high-risk pregnant women. Results: The incidence of fetal distress and low Apgar score in high-risk pregnancy and blood was low, and the incidence of fetal distress and low Apgar score were higher than those in normal group with high H P L, the difference was significant Sex. Conclusion: The method is specific, sensitive, fast, accurate, etc., can be used as a reliable indicator of placental function.