论文部分内容阅读
本文建立了新疆库鲁木提草中柚皮苷和木犀草素两种活性成分的高效液相色谱同时测定方法,并实现了野生及人工栽培库鲁木提草中柚皮苷和木犀草素的检测.采用Agilent HC-C18色谱柱(250×4.6mm,5μm)分离,以甲醇-0.2％HAc水溶液作为流动相,在柱温为室温和流速1.00 mL/min条件下进行梯度洗脱,实现了两种活性成分之间以及它们与干扰组分的良好分离.柚皮苷和木犀草素的线性范围分别为120～1 440mg/L(r=0.9998)和17.5～210 mg/L(r=0.9999),加标回收率分别为93.8％～100.2％(RSD=1.8％～2.7％,n=3)和98.3％～100.6％(RSD=1.3％～2.9％,n=3).研究结果表明野生库鲁木提草中柚皮苷和木犀草素的含量更高.“,”Here we developed an extraction method for the extraction of naringin and luteolin in Hymenolyma nana.The two active components in wild and cultivated samples were determined by high performance liquid chromatography(HPLC) with Agilent HC-C18 column(250 × 4.6 mm,5 μm),and the mobile phase of CH3OH-0.2％ HAc for the gradient elution with flow rate of 1.0 mL/min under room temperature.Naringin and luteolin were separated very well with each other and the coexisting components.Under the optimal chromatographic conditions,linear correlation were obtained in the concentration range of 120-1 440 mg/L(r=0.9998) for naringin and 17.5-210 mg/L(r=0.9999) for luteolin.The recoveries of naringin and luteolin were 93.8％-100.2％ (RSD=1.8％-2.7％,n=3) and 98.3 ％-100.6 ％ (RSD =1.3 ％-2.9 ％,n =3),respectively.These results illustrated that their content in wild Hymenolyma nana are higher than that in cultivated sample.The method achieved successful determination of naringin and luteolin in Hymenolyma nana,and the results could provide the guidance for the quality assessment,development and utilization of Hymenolyma nana.