论文部分内容阅读
目的:探讨抗p185c-erbB-2/neu基因工程抗体联合紫杉醇促进p185过表达的人乳腺癌细胞系BT474凋亡的效应及其机制。方法:采用MTS法检测基因工程抗体联合紫杉醇对BT474细胞增殖的抑制作用。用AnnexinV-FITC/PI法检测BT474细胞的凋亡率;以流式细胞术(FCM)分析DNA含量及细胞周期分布;用EMSA分析NF-κB的活化水平。结果:基因工程抗体联合紫杉醇对BT474细胞增殖的抑制作用具有协同效应,并可诱导细胞凋亡,将细胞阻滞在G1期;并可显著抑制BT474细胞中NF-κB的活化。结论:紫杉醇诱导乳腺癌细胞BT474凋亡的同时,可活化NF-κB。基因工程抗体联合紫杉醇是通过抑制NF-κB的活化而增强紫杉醇诱导癌细胞凋亡的作用。
AIM: To investigate the effect of anti-p185c-erbB-2 / neu engineered antibody combined with paclitaxel on the apoptosis of human breast cancer cell line BT474 overexpressing p185 and its mechanism. Methods: The inhibitory effect of genetically engineered antibody combined with paclitaxel on the proliferation of BT474 cells was detected by MTS assay. The apoptosis rate of BT474 cells was detected by Annexin V-FITC / PI. The DNA content and cell cycle distribution were analyzed by flow cytometry (FCM). The activation level of NF-κB was analyzed by EMSA. Results: Gene-engineered antibody combined with paclitaxel had a synergistic effect on the inhibition of BT474 cell proliferation and induced apoptosis, arresting cells at G1 phase and significantly inhibiting NF-κB activation in BT474 cells. Conclusion: Paclitaxel can induce NF-κB activation in breast cancer cell line BT474. Gene-engineered antibody combined with paclitaxel enhances paclitaxel-induced cancer cell apoptosis by inhibiting the activation of NF-κB.