根据人SRY基因序列,设计合成一对引物,在男性DNA中特异扩增一条422 bp的DNA片段,而女性中无任何扩增带出现。进一步将该片段克隆,限制性内切酶分析和测序表明,扩增片段就是SRY基因的保守区。随后利用其作探针进行分子杂交,在男性DNA中获得一个约12kb的带,与文献相一致。因此,利用该引物或克隆片段,在临床上可用于性连锁遗传病的产前诊断和性分化异常个体的研究。 According to the human SRY gene sequence, a pair of primers was designed and synthesized, and a 422 bp DNA fragment was amplified specifically in male DNA without any amplification band in the female. Further cloning of the fragment, restriction endonuclease analysis and sequencing showed that the amplified fragment was the conserved region of the SRY gene. Subsequent molecular hybridization was used as a probe to obtain a band of approximately 12 kb in male DNA, consistent with the literature. Therefore, the use of the primers or cloned fragments can be clinically used for genetic linkage disorder of prenatal diagnosis and sexual differentiation of abnormal individuals.