Embryogenic cell suspension was obtained fromthe calli developed from mature seeds of riceRoncarolo(Oryza sativa L., a japonica cultivarfrom Italy). The protoplasts were isolated fromcell suspension by treatment of enzyme mixtureand suspended in the solution containing 0. 56% Embryogenic cell suspension was obtained from the calli developed from mature seeds of rice Roncarolo (Oryza sativa L., a japonica cultivar from Italy). The protoplasts were isolated from cell suspension by treatment of enzyme mixture and suspended in the solution containing 0.56%