论文部分内容阅读
目的:通过检测实验性牙周炎大鼠牙龈组织及外周血中NO的变化,探讨大黄素治疗牙周炎的机制。方法:选取纯种雌性SD大鼠随机分为4组,各30只;正常对照组(N组)、牙周炎组(P组)、低浓度大黄素治疗组(PL组)及高浓度大黄素治疗组(PH组)。建立牙周炎动物模型并按分组用药,分别于4、6、8周采用股动脉放血法处死大鼠,留取血清,检测血清中一氧化氮(NO)水平。同时取一侧上颌骨做光镜形态学观察,取另一侧上颌骨结扎牙周围的牙龈组织测量NO含量,并作统计学分析。结果:PL和PH组大鼠的牙龈组织及外周血中的NO在实验的各阶段均明显低于P组(P<0.05)。结论:大黄素可以通过降低牙周炎大鼠牙龈及外周血中NO的含量,对牙周炎有一定的治疗意义。
Objective: To investigate the mechanism of emodin in the treatment of periodontitis by detecting the changes of NO in gingival tissues and peripheral blood of experimental periodontitis rats. Methods: Thirty SD female SD rats were randomly divided into 4 groups (30 rats in each group). The normal control group (N group), periodontitis group (P group), low concentration emodin group (PL group) and high concentration rhubarb Su treatment group (PH group). Animal models of periodontitis were established and divided into groups. Rats were sacrificed at 4, 6, and 8 weeks respectively by femoral artery perfusion method. Serum was collected and the level of serum nitric oxide (NO) was measured. At the same time take the side of the maxilla to do light microscope morphology, take the other side of the maxillary ligament around the gingival tissue measurement of NO content, and for statistical analysis. Results: The levels of NO in gingival tissues and peripheral blood in PL and PH groups were significantly lower than those in P groups at all stages (P <0.05). Conclusion: Emodin can reduce the content of NO in the gingival and peripheral blood of rats with periodontitis, and has certain therapeutic effect on periodontitis.