腺病毒伴随病毒(AAV)Rep基因编码蛋白对异种系统有双向调节作用。本文研究了Rep78蛋白对HIV-1在人源细胞293、HeLa和JW细胞内基因表达及病毒增殖释放的调控。作者使用转染技术进行研究。他们将不同质粒:包括含编码Rep78蛋白基因的pHIVrep;AAV2rep基因1033位点上核苷酸变异导致Rep蛋白产生显著下降的pHIV-repam;含 Adenovirus-associated virus (AAV) Rep gene encodes a protein that has a bi-directional regulatory effect on xenogenic systems. In this paper, the regulation of gene expression of Rep78 on HIV-1 gene expression in human 293, HeLa and JW cells and the release of virus were investigated. The authors used transfection techniques to study. They used different plasmids, including pHIVrep1, which contains the Rep78 protein gene; pHIV-repam, which causes a significant decrease in Rep protein due to nucleotide variation at the 1033 site of the AAV2rep gene;