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目的:研究甲基苯丙胺(MA)对大鼠心脏、肝脏和肾脏的毒性损伤以及氧化应激在其中的作用。方法:大鼠进行急性MA(10 mg·kg~(-1))给药,每天给药2次,4天后处死,测量心脏、肝脏、肾脏重量;运用HE染色对各器官组织进行形态学观察;检测血清中丙二醛(MDA)以及超氧化物歧化酶(SOD)的含量。结果:急性高剂量MA给药造成大鼠心脏和肾脏的膨大,肝脏细胞排列紊乱;引起心肌组织细胞纤维化、水肿、变性等;肾小球呈现萎缩、肾小管变性退化;同时,器官损伤伴随MDA含量显著上升和SOD活性显著下降(p<0.01)。结论:急性高剂量MA给药引起大鼠心脏、肾脏和肝脏明显毒性损伤,并伴随氧化应激水平的增高。
Objective: To study the toxic injury induced by methamphetamine (MA) in rat heart, liver and kidney and the role of oxidative stress in it. Methods: The rats were administered with acute MA (10 mg · kg -1) once daily for 2 times and sacrificed after 4 days. The heart, liver and kidney weights were measured. The morphological changes of each organ were observed by HE staining Serum malondialdehyde (MDA) and superoxide dismutase (SOD) levels were measured. Results: Acute high dose of MA resulted in enlargement of heart and kidney, disorganization of liver cells, fibrosis, edema and degeneration of myocardial cells, glomerular atrophy and degeneration of renal tubules. At the same time, MDA content increased significantly and SOD activity decreased significantly (p <0.01). Conclusion: Acute high dose of MA can cause obvious toxic injury in rat heart, kidney and liver accompanied with the increase of oxidative stress.