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目的:研究清热解毒剂的急性毒性、镇痛、抗炎作用。方法:采用最大耐受量(MTD)法研究其急性毒性,通过热板法和扭体法观察清热解毒剂的镇痛作用。采用二甲苯致小鼠耳肿胀、冰醋酸致小鼠毛细血管通透性增高、棉球致肉芽肿等方法观察该药的抗炎作用。结果:清热解毒剂MTD相当于人临床日用量的222倍(人每日用药量为0.9 g.kg-1);清热解毒剂中剂量对小鼠醋酸所致扭体反应有明显的镇痛作用;各剂量对对热板法引起的疼痛有不同程度的镇痛作用,其中中剂量镇痛作用的持续时间最长,药后120 min仍有明显的镇痛作用(P<0.05);中剂量对二甲苯致小鼠耳廓肿胀及醋酸致小鼠腹腔毛细血管通透性的增高有明显的抑制作用(P<0.05);各剂量对棉球致小鼠肉芽肿没有抑制作用。结论:清热解毒剂毒性较低,具有一定的镇痛作用,对急性炎症有抑制作用。
Objective: To study the acute toxicity, analgesic and anti-inflammatory effects of Qingrejiedu. Methods: The acute toxicity was studied by maximum tolerated dose (MTD) method. The analgesic effect of heat-clearing antidote was observed by hot plate method and writhing method. Xylene-induced mouse ear swelling, glacial acetic acid induced capillary permeability in mice, granuloma caused by cotton balls and other methods to observe the anti-inflammatory effect of the drug. Results: MTD of Qingrejiedu was equivalent to 222 times of daily clinical dosage (0.9 g.kg-1 daily for human); Qingrejiedu middle dose had obvious analgesic effect on mice writhing reaction induced by acetic acid ; Each dose on the hot plate pain caused by varying degrees of analgesic effect, including the longest duration of analgesic effect in the dose, 120 min after the drug is still significant analgesic effect (P <0.05); medium dose P-xylene induced mouse ear swelling and acetic acid-induced increased peritoneal capillary permeability of mice significantly inhibited (P <0.05); the dose of cotton balls caused no inhibition of mouse granuloma. Conclusion: Qingrejiedu low toxicity, has some analgesic effect, inhibition of acute inflammation.