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我们自1974年12月到1976年12月,收集到肝癌切除标本21例,对其中10例临床早期原发性肝癌(简称早期肝癌,患者术前无明确症状和体征,只是在肝病普查中因发现甲胎蛋白阳性而拟诊肝癌,后经病理切片证实者,癌块直径在0.5至4.5厘米之间)的超微结构进行了重点研究。现小结如下:材料和方法电镜肝癌标本均选择肿瘤、肿瘤边缘与非肿瘤交界处。取材后即用磷酸缓冲液配制的5%戊二醛及1%锹酸双固定。逐级丙酮脱水。环氧树脂618包埋。LKB8800lll型超薄切片机切片(4Onm或50nm)。醋酸铀及构橡酸铅染色。DAX3一8型等电子显微镜(简称电镜)观察。观察结果我们所观察到的早期原发性肝癌(主要是肝细胞癌)相当于光镜下肝细胞癌分级的工~n级,少数癌组织区域内也可见到肝细胞癌的IV级。早期肝癌细胞的变化主要表现在细胞器的排列紊乱、数量的增减、形态的异常。
From December 1974 to December 1976, we collected 21 specimens of hepatocellular carcinoma resection, and 10 of them were clinically early primary hepatocellular carcinoma (abbreviated as early hepatocellular carcinoma. The patients had no clear symptoms and signs before surgery, but only in the liver disease survey. The ultrastructure of tumors that were positive for alpha-fetoprotein and were diagnosed as liver cancer, confirmed by pathological section, and between 0.5 and 4.5 centimeters in diameter was investigated. The summary is as follows: Materials and Methods Electron microscopy The tumors, tumor margins, and non-tumor junctions were all selected for the liver cancer specimens. After the samples were taken, they were double-fixed with 5% glutaraldehyde and 1% citric acid prepared with phosphate buffer. Progressive acetone dehydration. Epoxy 618 is embedded. LKB8800lll ultra-thin slicer (4Onm or 50nm). Uranyl acetate and lead acetate dyeing. DAX3-8 type electron microscope (short for electron microscope) observation. Observed results We observed early stage primary liver cancer (mainly hepatocellular carcinoma) equivalent to light microscopy hepatocellular carcinoma graded work-n level, a small number of cancerous tissue areas can also be seen in hepatocellular carcinoma grade IV. The changes of early hepatoma cells are mainly manifested in disordered arrangement of organelles, increased or decreased number, and abnormal morphology.