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研究人参中甾醇成分,同时测定不同产区人参中总甾醇的含量。采用皂化法提取,用石油醚萃取得到甾醇,经过硅胶柱层析分离单体甾醇;采用分光光度法测定人参样品中总甾醇的含量。从人参中分离出2种单体甾醇,分别鉴定为-谷甾醇和豆甾醇;5个产区总甾醇含量均值为0.35%±0.10%。从人参中分离出-谷甾醇和豆甾醇,并首次测定5个产区总甾醇含量,为提升人参产业的进一步发展提供科学依据。
Study ginseng sterol composition, simultaneous determination of ginseng in different areas total sterol content. The saponification method was used to extract the sterols. The sterols were separated by silica gel column chromatography. The content of total sterols in the samples of ginseng was determined by spectrophotometry. Two kinds of monomeric sterols were isolated from Panax ginseng and identified as sitosterol and stigmasterol respectively. The average content of total sterols in the five producing areas was 0.35% ± 0.10%. Isolation of sitosterol and stigmasterol from ginseng and determination of total sterols content in 5 producing areas for the first time provided scientific basis for further development of ginseng industry.