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运用多核细胞法及胞质分裂阻断微核（ＣＢＭＮ）法对５组Ｗｉｓｔａｒ雄性大鼠外周血淋巴细胞ＨＰＲＴ基因位点突变频率及微核进行检测。实验组静脉注射放射性比活度为３．６４×１０５Ｂｑ／ｍｌ的晚期混合裂变产物后１至９ｄ心脏穿刺取血。结果表明：静注后第１ｄ，累积剂量达１．７３ｃＳｖ时，与对照组相比，ＨＰＲＴ位点突变频率、、微核率均已显著增加（ｐ＜０．０１）；累积剂量与ＨＰＲＴ位点突变频率、微核细胞率、微核率间均可拟合成剂量效应函数Ｙ＝ａ＋ｂｌｎＸ；ＨＰＲＴ基因突变检测可望成为生物剂量计。
The frequency and micronuclei of HPRT gene loci in peripheral blood lymphocytes of 5 Wistar male rats were detected by multinucleated cell method and cytokinesis-block micronucleus (CBMN) method. Experimental group intravenous radioactivity specific activity of 3.64 × 105Bq / ml advanced mixed fission products from 1 to 9d after cardiac puncture blood. The results showed that the frequency of HPRT mutation and micronucleus rate were significantly increased (p <0.01) at 1 day after intravenous injection when the cumulative dose reached 1.73 cSv. Compared with the control group, the cumulative dose and HPRT The frequency of point mutation, the rate of micronuclei, and the rate of micronucleus can be fitted to the dose-response function Y = a + blnX; the detection of HPRT mutation is expected to be a biological dosimeter.