将一个甘蓝型黄籽油菜RAPD标记(S1130)发展成显性SCAR标记(SCS1130)。比较SCS1130的三种检测方法,包括测定PCR反应物浓度、PCR板EB直接染色和电泳,结果表明EB直接染色是最简单快速的检测方法,可降低成本、省时。SCS1130的发展为开展甘蓝型黄籽油菜分子标记辅助选择创造条件,将便利和加快其选育。 A Brassica napus RAPD marker (S1130) was developed to a dominant SCAR marker (SCS1130). Comparison of SCS1130 three detection methods, including the determination of PCR reaction concentration, PCR plate EB direct staining and electrophoresis, the results show that EB direct staining is the most simple and rapid detection method, can reduce costs and save time. The development of SCS1130 will create the conditions for molecular marker-assisted selection of Brassica napus, which will facilitate and speed up its breeding.