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Objective:To investigate the effect of Jinmaitong(筋脉通,JMT)serum on the proliferation of rat Schwann cells(SCs)primarily cultured in high glucose medium.Method:SOs were primarily cultured in Dulbecco’s minmum essential medium(DMEM control),50 mmol/L glucose medium(50 mmol/L Glu),75 mmol/L glucose medium(75 mmol/L Glu),as well as 50 mmol/L glucose medium,with different concentrations of JMT serum(undiluted,1:2 diluted and 1:8 diluted)and Neurotropin(Ntp), respectively.The proliferation of SCs under different conditions was detected by MTT.Result:SCs grew exuberantly in DMEM within 24-72 h,but slowed down at 96 h.The proliferation of SCs was inhibited in 50 mmol/L Glu and 75 mmol/L Glu after cultures of 48,72 and 96 h,which showed that both were significantly different compared to the control group(P<0.01).The inhibition was more significant in 75 mmol/L Glu than in 50 mmol/L Glu(P<0.05).Spearman’s rho analysis revealed that the proliferation of SCs had a negative correlation with the concentration of glucose(r=-0.471,P<0.01).Excluding the time factor,partial correlation showed similar results(r=-0.679,P<0.01).After 48 h,the proliferation of SCs increased significantly in JMT1:2 and Ntp compared with 50 mmol/L Glu(control 0.437±0.019,50 mmol/ L Glu 0.367±0.035,JMT1:2 0.426±0.024,Ntp 0.422±0.013;P<0.01),and there were no statistically significant differences among the JMT groups,the Ntp group and the control group(P>0.05).Conclusions: The proliferation of SCs was inhibited in high glucose medium,and the inhibition was reduced by different concentrations of JMT serum,especially at JMT1:2.
Objective: To investigate the effect of Jinmaitong (on JMT) serum on the proliferation of rat Schwann cells (SCs) in cultured in high glucose medium. Method: SOs were incorporated in Dulbecco’s minmum essential medium (DMEM control), 50 (50 mmol / L Glu), 75 mmol / L glucose medium (75 mmol / L Glu), as well as 50 mmol / L glucose medium, with different concentrations of JMT serum and 1: 8 diluted) and Neurotropin (Ntp), respectively. The proliferation of SCs under different conditions was detected by MTT. Result: SCs grew exuberally in DMEM within 24-72 h, but slowed down at 96 h. The proliferation of SCs was inhibited in 50 mmol / L Glu and 75 mmol / L Glu after cultures of 48, 72 and 96 h, which showed both were significantly different compared to the control group (P <0.01). The inhibition was more significant in 75 mmol / L Glu than in 50 mmol / L Glu (P <0.05). Spearman’s rho analysis revealed that the proliferation of SCs had a negative correlation with t After 48 h, the proliferation of SCs increased significantly in JMT1: 2 (r = -0.471, P <0.01) .Excluding the time factor, partial correlation showed similar results and Ntp compared with 50 mmol / L Glu (control 0.437 ± 0.019, 50 mmol / L Glu 0.367 ± 0.035, JMT1: 2 0.426 ± 0.024, Ntp 0.422 ± 0.013; P <0.01), and there were no significant significant differences among the JMT groups, the Ntp group and the control group (P> 0.05). Conclusions: The proliferation of SCs was inhibited in high glucose medium, and the inhibition was reduced by different concentrations of JMT serum, especially at JMT1: 2.